Journal: Journal of Inflammation (London, England)

Article Title: Tissue-plasminogen activator effects on the phenotype of splenic myeloid cells in acute inflammation

doi: 10.1186/s12950-024-00375-0

Figure Lengend Snippet: References of flow cytometry antibodies used for immunophenotyping

Article Snippet: MHCII , REA813 , FITC , 130–112-386 , human IgG1/REA293 , Miltenyi Biotec.

Techniques: Flow Cytometry, Clone Assay

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD105 , REA794 , 50 , 130-112-169 , FITC , Miltenyi Biotec.

Techniques: Imaging

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Deep spatial profiling of human palatine tonsil tissues. (A) Hematoxylin and eosin (H&E) staining after 92 MICS cycles, including the marked epithelium, germinal center (GC), and T cell zone of the lymphoid follicle. MICS DAPI and stroma staining depicting the composition and structure of the tonsil. Markers: Collagen III, collagen IV, fibronectin (all extracellular matrix (ECM), cytokeratin (epithelium), podoplanin (lymphatic vessels), CD105/SM Actin (blood vessels). (B) Immune cell content of a human palatine tonsil comprising T cells (CD3), B cells (CD19/CD20), plasma cells (PCs) (CD38/CD138), NK cells (CD56), granulocytes (CD15/CD66b), mast cells (CD117), macrophages (MΦ) (CD163/CD169/CD206), myeloid dendritic cells (mDCs) (CD11c), and plasmacytoid dendritic cells (pDCs) (CD123). (C) Detailed view on the T cell zone, mainly composed of CD4 + helper T cells (T h ) and CD8 + cytotoxic T cells (T c ), mDCs (CD11c), and PCs (CD38/CD138). (D) Detailed view on the GC-mantle zone border, showing different B cells (CD11b, CD21, CD22), mDCs (CD11c), and PCs (CD38/CD138). (E) Cell annotations of three different tonsil samples plus respective bar graphs of gated cell populations, comparing the cell content between the three tonsil samples. Depicted markers and annotated cell types as indicated by the color code. ROI sizes: 976 x 640 µm, zoomed-in subregions in (C, D) : 334 µm x 219 µm. Scale bar: 100 µm.

Article Snippet: CD105 , REA794 , 50 , 130-112-169 , FITC , Miltenyi Biotec.

Techniques: Staining, Clinical Proteomics

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immuno-oncological description and characterization of cancerous tissues. For all three tumor samples, DAPI, stroma- and tumor-characterizing images (collagen III, collagen IV, cytokeratin, fibronectin, podoplanin, CD105/SM Actin), immune cell content in a 15-color image, as well as cell type annotation images and bar graphs are shown. (A) represents a colorectal carcinoma (CRC), (B) a prostate carcinoma (PCa), and (C) an intrahepatic cholangiocellular carcinoma (CCC). Depicted markers and annotated cell types as indicated by the color code. ROI sizes: (A) : 975 x 747 µm, (B) : 974 x 747 µm, (C) : 974 x 769 µm. Scale bar: 100 µm.

Article Snippet: CD105 , REA794 , 50 , 130-112-169 , FITC , Miltenyi Biotec.

Techniques:

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Detection of structural, immune, and tumor markers in three different hepatocellular carcinoma (HCC) tumor regions. (A) H&E staining after 92 MICS cycles plus marked regions of interest (ROIs). (B) Pre-run DAPI staining for ROI definition. (C–E) represent tumor-associated stroma area, tumor margin, and tumor core. For each ROI, images for stroma and tumor characterization (collagen III, collagen IV, cytokeratin, fibronectin, podoplanin, CD105/SM Actin), as well as immune cells (T cells, B cells, PCs, mDCs, pDCs, mast cells, MΦ, myeloid-derived suppressor cells (MDSCs)), and the cell type annotation are shown. (F) Quantifications of the annotated cell types by bar graphs are outlined for each ROI. Depicted markers and annotated cell types as indicated by the color code. ROI sizes: Tumor-associated stroma (ROI14): 975 x 770 µm, tumor margin (ROI15): 975 x 769 µm, tumor core (ROI16): 975 x 769 µm. Scale bar: 100 µm.

Article Snippet: CD105 , REA794 , 50 , 130-112-169 , FITC , Miltenyi Biotec.

Techniques: Staining, Derivative Assay